Fig. 3
From: Systematic evaluation of characteristics of the membrane-based fed-batch shake flask
Analysis of glucose concentrations at three different positions in the reservoir system in E. coli fed-batch cultivations. Samples were taken at the upper liquid surface (top, filled circles), from the middle of the glass tube (middle, open squares) and behind the membrane (bottom, filled triangles) during cultivations for varied initial reservoir glucose concentrations (250, 375, 500 and 750 g/L); cultivation conditions: Wilms-MOPS-mineral medium (0.2 M MOPS), temperature: 37 °C, shaking frequency: 350 rpm, shaking diameter: 50 mm, culture broth volume: 10 mL, inoculation OD600: 0.5, reservoir filling volume: 2 mL, dialysis membrane: Reichelt (1) dialysis membrane, membrane area: 18.1 mm2; strain: E. coli BL21 (DE3) pRhotHi-2-EcFbFP