Fig. 2
From: Systematic evaluation of characteristics of the membrane-based fed-batch shake flask
Effect of initial reservoir glucose concentration on oxygen transfer rate of E. coli fed-batch cultivations (duplicates). a For clarity reasons the cultivations with 75, 125 and 500 g/L initial reservoir glucose concentration have been shifted timewise as indicated, due to deviating lag-phases. b The oxygen transfer rate level of the fed-batch phase is respectively plotted over the initial reservoir glucose concentration; cultivation conditions: Wilms-MOPS-mineral medium (0.2 M MOPS), temperature: 37 °C, shaking frequency: 350 rpm, shaking diameter: 50 mm, culture broth volume: 10 mL, inoculation OD600: 0.5, reservoir filling volume: 2 mL, dialysis membrane: Reichelt (1) dialysis membrane, membrane area: 18.1 mm2; strain: E. coli BL21 (DE3) pRhotHi-2-EcFbFP