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Table 4 Primers used to generate fragments for cloning by PCR amplification

From: Scaling up and scaling down the production of galactaric acid from pectin using Trichoderma reesei

Product

Primer

Sequence

Product size (bp)

gar1 5′flank (tre22004)

MU01_gar1_5f_for

GGTAACGCCAGGGTTTTCCCAGTCACGACGGTTTAAACTTATATCCACCGTGTCCCAG

1610

MU02_gar1_5f_rev

GACGCAGTTGTTTGAGCAAC

trpC terminator (Aspergillus)

MU03_trpCt_for

GTGGATAACCCCATCTTCAAGCAGTCCTGAGATCCACTTAACGTTACTGAAATCA

803

MU04_trpCt_rev

GAGTGGAGATGTGGAGTGGG

gar1 3′direct repeat (tre22004)

MU05_gar1_3dr_for

TGTGTAAGCGCCCACTCCACATCTCCACTCGGCGCGCCTTGCATTGGTCAGAGCGGTA

361

MU06_gar1_3dr_rev

CGAGAGCAGAGCAGCAGTAGTCGATGCTAGGCGGCCGCCCGACTTGGAGAAGCTCGTC

gar1 3′flank (tre22004)

MU07_gar1_3f_for

CCAGCTGCGATTGATGTGTATCTTTGCATGGCGGCCGCTTGCATTGGTCAGAGCGGTA

1576

MU08_gar1_3f_rev

AGCGGATAACAATTTCACACAGGAAACAGCGTTTAAACAAGCAGTGGATGACTTGCTG

cbh1 promoter (tre123989)

MU09_cbh1p_for

AGGTTAGTAGGTTGCTCAAACAACTGCGTCGGCCGGCCTGTGGCAACAAGAGGCCAGA

1646

MU10_cbh1p_rev

GGCAGCGCCGGTGACGAGCAGGCGCTTCATGATGCGCAGTCCGCGGTTGA

cDNA1 promoter (tre123515)

MU11_cDNA1p_for

AGGTTAGTAGGTTGCTCAAACAACTGCGTCGGCCGGCCGAATTCGGTCTGAAGGACGT

1231

MU12_cDNA1p_rev

GGCAGCGCCGGTGACGAGCAGGCGCTTCATGTTGAGAGAAGTTGTTGGATTGA

pdc1 promoter (tre121534)

MU13_pdc1p_for

AGGTTAGTAGGTTGCTCAAACAACTGCGTCGGCCGGCCAAAGGAGGGAGCATTCTTCG

1370

MU14_pdc1p_rev

GGCAGCGCCGGTGACGAGCAGGCGCTTCATGATTGTGCTGTAGCTGCGCT

  1. The gene identifiers (tre-numbers) refer to Joint Genome Institute T. reesei assembly release version 2.0