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Table 4 Fermentation products for E. coli BL21 (DE3) harbouring plasmid pPET28_sbm

From: Awakening sleeping beauty: production of propionic acid in Escherichia coli through the sbm operon requires the activity of a methylmalonyl-CoA epimerase

 

Glucosea

Pyruvate

Succinate

Lactate

Formate

Acetate

Propionate

Ethanol

Biomassb

Mineral medium

22.93 ± 2.75

1.10 ± 0.07

1.88 ± 0.09

30.90 ± 3.39

11.66 ± 0.89

5.78 ± 0.32

ND

11.10 ± 0.28

0.33 ± .04

Complex medium

26.49 ± 2.54

0.64 ± 0.04

1.17 ± 0.10

33.59 ± 4.03

15.31 ± 0.68

7.47 ± 0.73

0.34 ± 0.02

11.56 ± 0.64

0.54 ± .08

  1. Data correspond to the average of two replicates. All concentration are reported in mM. ND: not detected or below the limit of detection
  2. a Data reported correspond to total glucose consumed out of 100 mM
  3. b Biomass is reported in g/L
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Microbial Cell Factories

ISSN: 1475-2859

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