Table 2 Primers
Primer name | Sequence | Description |
|---|---|---|
sbmF1 | CCTTAAGTCTAGAAGGAGAAAACCGATGTCTAACGTGCAGGAG | Forward primer for sbm region amplification |
sbmR1 | GCATGCCAAGCTTGCGGCCGCACTAGTTTATTAACCCAGCATCGAGCC | Reverse primer for sbm region amplification |
PBADF1 | GATATCAATCGATTCTAGATTATGACAACTTGACGGC | Forward primer for mce gene amplification |
PBADR1 | GATATCAAAGCTTGCGGCCGCTTATTACATCGGTTTTCTCCTACTAGTAGCTCGAATTCCCAAAAAAAC | Reverse primer for mce gene amplification |
cPBAD_F | TTCTCATGTTTGACAGCTTATCATCGATTC | Confirmation forward primer for PBAD region insertion in pBR322 |
cPBAD_R | CACGGTGCCTGACTGCGTTAG | Confirmation reverse primer for PBAD region insertion in pBR322 |
cPET-sbmF1 | CGAGCCCGATCTTCCCCATC | Confirmation forward primer for sbm region insertion in pET38a+ |
cPET-sbmR1 | TGGTCCACGGTTGGGCGG | Confirmation reverse primer for sbm region insertion in pET38a+ |
cPET-sbmF2 | GCGCCCGCACTATCATTG | Confirmation forward primer for sbm region insertion in pET38a+ |
cPET-sbmR2 | ATCCGGATATAGTTCCTCCTTTCAGC | Confirmation reverse primer for sbm region insertion in pET38a+ |
c_sbm-mceF1 | CCATGTTGATCACAGCGAAC | Confirmation forward primer for mce gene insertion in plasmid pET_sbm |
C_sbm-mceR1 | GTATTTCGGGTGCAGGAAGA | Confirmation reverse for mce gene insertion in plasmid pET_sbm |