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Table 1 Bacterial strains and plasmids used

From: Awakening sleeping beauty: production of propionic acid in Escherichia coli through the sbm operon requires the activity of a methylmalonyl-CoA epimerase

Strain/Plasmid

Description

Reference

Escherichia coli BL21 (DE3)

Expression host

Bioline

Escherichia coli DH5α

Cloning and expression host

Bioline

Escherichia coli MG1655

DNA template

CGSCa 7740

pET28a+

Cloning vector

LifeTechnologies

pBR322

Cloning vector

LifeTechnologies

pMA_mce

pMA derived plasmid. AmpR PT7-Pnar-mce-tT7

LifeTechnologies

pET28_sbm

pET28a+ derived plasmid. KanR-PT7-sbm-tT7

This work

pET28_sbm-mce

pET28a+ derived plasmid. KanR-PT7-sbm-mce-tT7

This work

pBRPBAD

pBR322 derived plasmid AmpR. araC-PBAD

This work

pBRPBAD_sbm

pBR322 derived plasmid AmpR. araC-PBAD-sbm

This work

pBRPBAD_sbm-mce

pBR322 derived plasmid AmpR. araC-PBAD-sbm-mce

This work

  1. a Purchased from the Coli Genetic Stock Centre (CGSC)