Fig. 7
Process scale up to 10 L shows comparable growth and production characteristics. a Growth characteristics of three cultivations in 10 L stirred tank reactors (I–III) in complex medium with maltose as carbon source at 30 °C. The employed strain was DHN-270 (Pccg1nr-glat-ht186-10xhis, exo-1, ∆spr-7, ∆apr-9, ∆apr-3, ∆NCU00263). The dissolved oxygen level and pH value (pH 6.5) were controlled as in the small scale experiments. b Production analysis of cultivation II. Proteins from the supernatant were separated via SDS-PAGE (S) and the product was detected after Western blot transfer (WB). As a negative control (K), a comparable production strain missing the his10-tag was used (DHN-281, Pccg1nr-glat-ht186-13xmyc, exo-1, ∆spr-7, ∆apr-9, ∆apr-3, ∆NCU00263). The signal was visualized directly on the membrane with an NBT/BCIP system. The protease assay (P) was performed as mentioned above. As a positive control, HT186-D11 (HT, 10 μg/mL) was used