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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Establishment of Neurospora crassa as a host for heterologous protein production using a human antibody fragment as a model product

Fig. 4

The pH value of the culture medium influences growth and the extracellular protease activity. a Growth characteristics and b protease activity in cultivations with different pH values. The production strain DHN-201 (Pvvd-glat-ht186-13×myc, exo-1, ∆vib-1, ∆vvd) was cultivated in a controlled 1 L bioreactor system in minimal Bird medium with maltose as the sole carbon source at 30 °C. The pH value was controlled at indicated levels by addition of sodium hydroxide throughout cultivation. For the protease activity, the before mentioned protease assay was performed, band intensities were determined via image processing software and ratios calculated in respect to an undigested HT186-D11. Subsequently, those values were divided by the cell dry mass. Due to different growth rates, the culture time had to be normalized. Therefore, the amount of produced CO2 at the maximal XCO2 value was calculated and defined as 100% (CO2, max). Hence, protease activity values at a certain abscissa value are comparable

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