Fig. 9From: Biomarkers allow detection of nutrient limitations and respective supplementation for elimination in Pichia pastoris fed-batch culturesRelative transcript levels of selected marker genes for sulfur-, phosphorus-, and nitrogen limitation in the reference and supplemented cultivations (1× SO4, 2× SO4, 1× PO4). Transcript levels are normalized to ACT1 housekeeping gene and transcript levels of the middle (27 h) and end of the methanol fed-batch (67 h) are further normalized to the glycerol fed-batch (FB)Back to article page