Fig. 1

Schematic representation of the fed-batch cultivations for transcriptional analysis of P. pastoris. The process comprised a glycerol batch phase, followed by a limiting glycerol fed-batch for 5 h to double the biomass. Then methanol-grown cultures received a methanol pulse, while glucose-grown cultures were slowly fed with glucose to accumulate the same amount of biomass during this period (Asterisk). Afterwards a constant methanol or glucose feed was applied