Eliminating acetate formation improves citramalate production by metabolically engineered Escherichia coli

Table 1 Batch fermentations with strains of E. coli having knockouts in genes associated with acetate formation and expressing citramalate synthase via the pZE12-cimA plasmid

Strain	Key gene deletions	Citramalate yield (g/g)	Acetate yield (g/g)	Volumetric productivity (g/L h)
MEC499/pZE12-cimA	gltA leuC ackA	0.585^a	0.030^a	0.27^a
MEC562/pZE12-cimA	gltA leuC ackA-pta	0.642^b	0.027^a	0.31^b
MEC596/pZE12-cimA	gltA leuC ackA poxB	0.609^a	0.007^c	0.33^c
MEC568/pZE12-cimA	gltA leuC ackA-pta poxB	0.626^a,b	0.015^b	0.34^d
MEC606/pZE12-cimA	gltA leuC ackA-pta poxB ppsA	0.604^a	0.023^a	0.33^c

The media for all experiments contained initially 30 g/L glucose, 5 g/L glutamate and 1 g/L leucine. Values in a column with different letters indicate significant difference at p < 0.05

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