Fig. 1

Metabolic pathways for citramalate synthesis in Escherichia coli expressing cimA coding citramalate synthase (blue). Key enzymes (and corresponding genes) are: citrate synthase (gltA), 3-isopropylmalate dehydratase (leuC), acetate kinase (ackA), phosphotransacetylase (pta), acetyl-CoA synthetase (acs), pyruvate oxidase (poxB), phosphoenolpyruvate synthase (ppsA). All strains examined in this study had deletions in the gltA, leuC and ackA genes as indicated in red