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Table 1 Substrate specificity and kinetic constants of LacH

From: Biochemical characterization of an enantioselective esterase from Brevundimonas sp. LY-2

Substrate Specific activity (μmol min−1 mg−1) k cat (s−1) K m (mM) k cat/K m (s−1 mM−1)
Lactofen 2.97 ± 0.61 0.87 ± 0.09 0.067 ± 0.002 12.9
p-Nitrophenyl acetate 118 ± 5.3 8.39 ± 0.28 0.147 ± 0.008 50.1
p-Nitrophenyl butyrate 88.1 ± 3.2 7.48 ± 0.45 0.185 ± 0.009 40.4
p-Nitrophenyl caproate 15.8 ± 1.2 3.47 ± 0.13 0.208 ± 0.012 16.7
p-Nitrophenyl caprylate 4.90 ± 0.18 0.767 ± 0.06 0.335 ± 0.016 2.29
p-Nitrophenyl laurate ND ND ND ND
  1. ND not detectable