Fig. 4

Effect of temperature on the activity (triangles) and stability (boxes) of LacH. The activity of the enzyme at different temperatures was measured in 50 mM sodium phosphate buffer (pH 7.0) using lactofen as the substrate. For determination of the thermostability, the enzyme was preincubated at various temperatures ranging from 15 to 80 °C for 30 min, and the residual activity was determined at pH 7.0 and 40 °C