An evolutionary optimization of a rhodopsin-based phototrophic metabolism in Escherichia coli

Table 1 Cell growths of rhodopsin-expressing E. coli strains with and without illumination

Strain	Description	Cell growth (OD₆₀₀)^a		Phototrophic growth Δ[B − A] (fold)
Strain	Description	In dark (A)	In light (B)	Phototrophic growth Δ[B − A] (fold)
W3110	Negative control	1.78 ± 0.05	1.78 ± 0.04	0^b
W3110/pKJ606-GR	Ancestral strain	1.52 ± 0.05	1.75 ± 0.08	0.23 (1.0)
ET5/pKJ606-GR	Evolved strain	1.52 ± 0.00	2.03 ± 0.08	0.51 (2.2)

^aData represent the mean of optical densities ± SD at 600 nm from at least three repeats
^bWild-type E. coli W3110 did not show any difference between growth in dark (A) and growth in light (B)

ISSN: 1475-2859