Fig. 4

Cell growth and lipid content of D12, D61 and D62 overexpressing strains. Strains were cultured in a 2 l fermenter with the 1.5 l K&R medium during 84 h at 30 °C, pH 4.5, stirred at 500 rpm, with aeration at 0.5 vvm. In the fermentation process, biomass and lipid content were determined every 12 h. a Cell dry weight, b lipid content of cell dry weight. Values were mean of three independent fermentation experiments. Error bars represent the standard error of the mean