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Table 2 Plasmids used in this study

From: Efficient soluble expression of disulfide bonded proteins in the cytoplasm of Escherichia coli in fed-batch fermentations on chemically defined minimal media

Plasmid

Details

Selection

References

pMJS144

MH6M-mature human GH1, coErv1p, coPDI

AmpR

This study

pMJS145

MH6M-mature human IL-6, coErv1p, coPDI

AmpR

This study

pYU25

MH6M-mature hen avidin, coErv1p, coPDI

AmpR

This study

pJV84

scFv human IgA1-GSH6

AmpR

[16]

pMJS205

coErv1p, coPDI

CmR

[16]

  1. All plasmids except pMJS205 were pET23/Ptac based. pMJS205 is pLysS/Ptac based