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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Efficient soluble expression of disulfide bonded proteins in the cytoplasm of Escherichia coli in fed-batch fermentations on chemically defined minimal media

Fig. 2

Production of scFv IgA1. a Representative growth profile of K-12 strain BW25113 co-expressing coPDI, coErv1p and scFv IgA1. Error bars represent the standard deviation from 3 samples. F indicates feeding started, I indicates induction. Cells were harvested and protein purified from the last time point shown. b Coomassie stained reducing SDS-PAGE analysis of produced proteins: molecular marker, total E. coli lysate (T), soluble protein fraction (S) and IMAC purified scFv. c Bradford analysis of sheared (+) and non-sheared (−) cells from two fermentations of scFv IgA1 (n = 6). There was no significant difference in protein concentration between sheared and non-sheared cells indicating the E. coli strains with CyDisCo components are robust

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