Skip to main content

Advertisement

Table 1 Strains used in this study

From: Cell foundry with high product specificity and catalytic activity for 21-deoxycortisol biotransformation

Strain Description Source
E. coli C43(DE3) F ompT gal hsdSB (rB- mB-) dcm Ion λ [47]
E. coli MG1655(DE3) MG1655 ΔendA ΔrecA (DE3), transformation strain [48]
S. cerevisiae MATα, his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 This lab
Y. lipolytica MATA, ura3-302, leu2-270, lys8-11, pex17-ha [49]
M. smegmatis mc2155 ept-1 mc26 mutant efficient for electroporation [50]
Ec02040101 E. coli MG1655(DE3) with plasmids pETXST39 (pET21a-Adx 4108 -Cyp11B1_Hs-AdR) and pGro7 This study
Ec02040102 E. coli MG1655(DE3) with plasmids pETXST40 (pET21a-Adx 4108 -Cyp11B1_Bt-AdR) and pGro7 This study
Ec02040103 E. coli MG1655(DE3) with plasmids pETXST41 (pET21a-Adx 4108 -Cyp11B1_Rn-AdR) and pGro7 This study
Ec02040104 E. coli MG1655(DE3) with plasmids pETXST42 (pET21a-Adx 4108 -Cyp11B1_Oa-AdR) and pGro7 This study
Ec02040105 E. coli MG1655(DE3) with plasmids pETXST43 (pET21a-Adx 4108 -Cyp11B1_Sh-AdR) and pGro7 This study
Ec02040106 E. coli MG1655(DE3) with plasmids pETXST44 (pET21a-Adx 4108 -Cyp11B1_Pa-AdR) and pGro7 This study
Ec02040107 E. coli MG1655(DE3) with plasmids pETXST45 (pET21a-Adx 4108 -Cyp11B1_Cco-AdR) and pGro7 This study
Ec02040108 E. coli MG1655(DE3) with plasmids pETXST46 (pET21a-Adx 4108 -Cyp11B1_Mo-AdR) and pGro7 This study
Ec02040109 E. coli MG1655(DE3) with plasmids pETXST47 (pET21a-Adx 4108 -Cyp11B1_Sa-AdR) and pGro7 This study
Ec02040110 E. coli MG1655(DE3) with plasmids pETXST48 (pET21a-Adx 4108 -Cyp11B1_HsG25R/G46R/L52M-AdR) and pGro7 This study
Ec02040111 E. coli MG1655(DE3) with plasmids pETXST49 (pET21a-Adx 4108 -Cyp11B1_BtG25R-AdR) and pGro7 This study
Ec02040112 E. coli MG1655(DE3) with plasmids pETXST50 (pET21a-Adx 4108 -Cyp11B1_RnG25R/G46R-AdR) and pGro7 This study
Ec02040113 E. coli MG1655(DE3) with plasmids pETXST51 (pET21a-Adx 4108 -Cyp11B1_OaG25R/G46R/V52M-AdR) and pGro7 This study
Ec02040114 E. coli MG1655(DE3) with plasmids pETXST52 (pET21a-Adx 4108 -Cyp11B1_ShT26R/S55R-AdR) and pGro7 This study
Ec02040115 E. coli MG1655(DE3) with plasmids pETXST53 (pET21a-Adx 4108 -Cyp11B1_PaG25R/G46R/A52M-AdR) and pGro7 This study
Ec02040116 E. coli MG1655(DE3) with plasmids pETXST54 (pET21a-Adx 4108 -Cyp11B1_CcoY20R/W38R/N44M-AdR) and pGro7 This study
Ec02040117 E. coli MG1655(DE3) with plasmids pETXST55 (pET21a-Adx 4108 -Cyp11B1_MoA30R/S50R/D56M-AdR) and pGro7 This study
Ec02040118 E. coli MG1655(DE3) with plasmids pETXST56 (pET21a-Adx 4108 -Cyp11B1_SaA30R/V41R/L47M-AdR) and pGro7 This study
Ec02040119 E. coli MG1655(DE3) with plasmids pETXST57 (pET21aAdx 4108 -Cyp11B1_HsG25R/G46R/L52M/R384A-AdR) and pGro7 This study
Ec02040120 E. coli MG1655(DE3) with plasmids pETXST58 (pET21a- Adx 4108 -Cyp11B1_Hs G25R/G46R/L52M/R110A-AdR) and pGro7 This study
Ec02040122 E. coli MG1655(DE3) with plasmids pETXST59 (pET21a-Adx 4108 -Cyp11B1_Hs G25R/G46R/L52M/F381A/L382S-AdR) and pGro7 This study
Ec02040123 E. coli MG1655(DE3) with plasmids pETXST60 (pET21a-Adx 4108 -Cyp11B1_Hs G25R/G46R/L52M/F381A/L382T-AdR) and pGro7 This study
Ec02040124 E. coli MG1655(DE3) with plasmids pETXST61 (pET21a-Adx 4108 -Cyp11B1_Hs G25R/G46R/L52M/I488L-AdR) and pGro7 This study
Ec02040125 E. coli MG1655(DE3) with plasmids pETXST62 (pET21a-Adx 4108 -Cyp11B1_Hs G25R/G46R/L52M/F381A/L382S/I488L-AdR) and pGro7 This study