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Table 2 Purification of recombinant αAI from culture supernatants of K. lactis YCT390/αAI-OPT strain 68A

From: Heterologous expression of an α-amylase inhibitor from common bean (Phaseolus vulgaris) in Kluyveromyces lactis and Saccharomyces cerevisiae

Purification step Total protein (μg) Specific inhibitory activity (IU/mg)
Dialysed and freeze-dried culture supernatant 899 ND
Anion-exchange batch-adsorption (DEAE-Sepharose CL-6B) 227 8
Affinity batch-adsorption (α-amylase-Sepharose) 8.45 234