Table 2 Purification of recombinant αAI from culture supernatants of K. lactis YCT390/αAI-OPT strain 68A
Purification step | Total protein (μg) | Specific inhibitory activity (IU/mg) |
|---|---|---|
Dialysed and freeze-dried culture supernatant | 899 | ND |
Anion-exchange batch-adsorption (DEAE-Sepharose CL-6B) | 227 | 8 |
Affinity batch-adsorption (α-amylase-Sepharose) | 8.45 | 234 |