Heterologous expression of an α-amylase inhibitor from common bean (Phaseolus vulgaris) in Kluyveromyces lactis and Saccharomyces cerevisiae

Table 1 Gene copy number and relative processing of recombinant αAI among strains of K. lactis YCT390/αAI-OPT

K. lactis strain/colony	Gene copy number		Relative processed αAI	Inhibitory activity (%)
K. lactis strain/colony	αAI-OPT	KlACT1	Relative processed αAI	Inhibitory activity (%)
Untransformed YCT390 (negative control)	0	1	–	10.3
YCT390/αAI-OPT 68A	6	1	++++	89.8
YCT390/αAI-OPT 17A	5	1	+++	84.6
YCT390/αAI-OPT 47B	6	1	++	59.2
YCT390/αAI-OPT 29B	6	1	+	49.5
YCT390/αAI-OPT 13B	6	1	–	16.6

Estimated copy number of genes αAI-OPT and KlACT1 (control) in genomic DNA of selected strains of K. lactis YCT390/αAI-OPT measured by q-PCR. Relative amount of processed recombinant αAI and percentage of inhibitory activity against porcine pancreatic α-amylase among pre-purified extracts of K. lactis YCT390/αAI-OPT strains. Untransformed strain YCT390 was used as negative control

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