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Table 1 Gene copy number and relative processing of recombinant αAI among strains of K. lactis YCT390/αAI-OPT

From: Heterologous expression of an α-amylase inhibitor from common bean (Phaseolus vulgaris) in Kluyveromyces lactis and Saccharomyces cerevisiae

K. lactis strain/colony Gene copy number  Relative processed αAI Inhibitory activity (%)
Untransformed YCT390 (negative control) 0 1 10.3
YCT390/αAI-OPT 68A 6 1 ++++ 89.8
YCT390/αAI-OPT 17A 5 1 +++ 84.6
YCT390/αAI-OPT 47B 6 1 ++ 59.2
YCT390/αAI-OPT 29B 6 1 + 49.5
YCT390/αAI-OPT 13B 6 1 16.6
  1. Estimated copy number of genes αAI-OPT and KlACT1 (control) in genomic DNA of selected strains of K. lactis YCT390/αAI-OPT measured by q-PCR. Relative amount of processed recombinant αAI and percentage of inhibitory activity against porcine pancreatic α-amylase among pre-purified extracts of K. lactis YCT390/αAI-OPT strains. Untransformed strain YCT390 was used as negative control