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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Heterologous expression of an α-amylase inhibitor from common bean (Phaseolus vulgaris) in Kluyveromyces lactis and Saccharomyces cerevisiae

Fig. 5

Inhibition of α-amylase by extracts from recombinant strains of K. lactis. Inhibition of porcine pancreatic α-amylase by a partially purified culture supernatants (300 µg of protein) and b lysed-cell extracts (500 µg of protein) from five colonies of K. lactis YCT390/αAI-OPT strains 13B, 68A, 17A, 47B and 29B. Untransformed strain YCT390 was used as negative control (−C) and pure Pinto-αAI (200 ng) was used as positive control (+C). Culture supernatants were partially purified with DEAE-Sepharose prior to inhibitor activity assays. Results are expressed as inhibition units (IU). Data from triplicate experiments were expressed as the average ± standard error

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