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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Heterologous expression of an α-amylase inhibitor from common bean (Phaseolus vulgaris) in Kluyveromyces lactis and Saccharomyces cerevisiae

Fig. 3

Western blot and α-amylase inhibitory activity of proteins from selected colonies of S. cerevisiae YPH499 with αAI-OPT. a Western blot of proteins (25 µg) from culture supernatants and lysed-cells of colonies 8, 27 and 92 transformed with αAI-OPT; the parent strain YPH499 transformed with empty plasmid pYES2 was used as negative control (−C) and pure Pinto-αAI (200 ng) was used as positive control (+C). Molecular markers are shown in kilodaltons at left. b Inhibitory activity against porcine pancreatic α-amylase of lysed-cell extracts (100 µg of protein) of S. cerevisiae YPH499/pYES2-αAI-OPT colonies 8, 27 and 92. Parent strain YPH499 transformed with empty plasmid pYES2 was used as a negative control (−C) and pure Pinto-αAI (200 ng) was used as a positive control (+C). Results are expressed as inhibition units (IU). Data from triplicate experiments were expressed as the average ± standard error. ***P < 0.05 as compared to S. cerevisiae YPH499/pYES2

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