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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Heterologous expression of an α-amylase inhibitor from common bean (Phaseolus vulgaris) in Kluyveromyces lactis and Saccharomyces cerevisiae

Fig. 1

Schematic representation of the gene constructs used for αAI production. Diagrams of a the native αAI gene from cv. Pinto comprising signal peptide (SP), α- and β- subunits and the propeptide sequences; b the synthetic αAI-OPT gene, optimised for expression in two yeasts, including a Kozak consensus sequence and flanked by HindIII and NotI restriction sites. Diagrams of constructs c pKLAC2-αAI-OPT and d pYES2-αAI-OPT. PLac4-PBI and PGal1 correspond to promoters Lac4-PBI and Gal1 in pKLAC2 and pYES2, respectively. TTLac4-PBI and TTCYC correspond to 3′ terminators Lac4-PBI and CYC in pKLAC2 and pYES2, respectively

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