Table 3 Deduced functions of genes in the nocamycin biosynthetic gene cluster
Gene | Length (amino acids) | Closest similar protein accession number, origin, identity/similarity (%) | Deduced function |
|---|---|---|---|
Orf1 | 306 | KOX27604, Saccharothrix sp. NRRL B-16348 | Short-chain dehydrogenase |
Ofr2 | 664 | KOX27605.1, Saccharothrix sp. NRRL B-16348, 81/86% | Helicase |
Orf3 | 455 | WP_053719771, Saccharothrix sp. NRRL B-16348 | Hypothetical protein |
NcmM | 225 | SCD95979, Streptomyces sp. PalvLS-984, 68/79% | Transcriptional regulator |
NcmL | 511 | EJI98707, Rhodococcus sp. JVH1, 50/60% | Monooxygenase |
NcmN | 911 | AFI57028 (QmnRg4), Amycolatopsis orientalis, 43/55% | LuxR family regulator |
NcmO | 412 | AFI57027 (QmnO), Amycolatopsis orientalis, 48/63% | Cytochrome P450 oxidase |
NcmP | 287 | ACN29714 (NokK), Nonomuraea longicatena, 44/55% | Carboxylate O-methyltransferase |
NcmC | 272 | ADY38535 (TrdC), Streptomyces sp. SCSIO1666, 45/58% | Dieckmann cyclase |
NcmB | 1119 | ADY38536 (TrdD), Streptomyces sp. SCSIO1666, 56/66% | Non-ribosomal peptide synthetase |
NcmD | 271 | AII10529, Rhodococcus opacus, 45/60% | Short-chain dehydrogenase |
NcmQ | 120 | EJY55702, Alicyclobacillus hesperidum URH17-3-68, 41/55% | Glyoxalase/bleomycin resistance protein |
NcmAI | 4915 | CBA11584 (SlgA1), Streptomyces lydicus, 54/64% | Type I polyketide synthase |
NcmAII | 1786 | EHY88978, Saccharomonospora azurea NA-128, 54/65% | Type I polyketide synthase |
NcmAIII | 1554 | CCF23202, Streptomyces hygroscopicus, 60/69% | Type I polyketide synthase |
NcmAIV | 1786 | CCF23202.1, Streptomyces hygroscopicus, 56/66% | Type I polyketide synthase |
NcmAV | 2679 | AEP40935.1, Nocardiopsis sp. FU40, 50/60% | Type I polyketide synthase |
NcmE | 273 | ADC79643 (TamE), Streptomyces sp. 307-9, 60/76% | Glycoside hydrolase |
NcmF | 487 | ADY38538 (TrdF), Streptomyces sp. SCSIO1666, 50/61% | Prenyltransferase |
NcmG | 397 | ADZ45320(Mur7), Streptomyces sp. NRRL 30471, 51/64% | Cytochrome P450 oxidase |
NcmH | 543 | CAH10178 (ChaT1), Streptomyces chartreusis, 42/60% | Multiple drug transporter |
NcmI | 197 | KKZ83567, Rhizobium phaseoli Ch24-10, 41/61% | PadR family transcriptional regulator |
NcmJ | 713 | WP_037345636, Sciscionella sp. SE31, 69/79% | AAA family ATPase |
NcmK | 213 | ADY38543(TrdK) Streptomyces sp. SCSIO1666, 49/64% | TetR family transcriptional regulator |
Orf4 | 110 | GAT66653, Planomonospora sphaerica, 43/54% | Ohr subfamily peroxiredoxin |
Orf5 | 232 | GAT10151, Mycobacterium novocastrense, 64/73% | Ubiquinone biosynthesis methyltransferase UbiE |
Orf6 | 322 | KDO05396, Amycolatopsis mediterranei, 68/77% | (2Fe–2S) ferredoxin |