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Table 1 Bacteria and plasmids used in this study

From: Identification of nocamycin biosynthetic gene cluster from Saccharothrix syringae NRRL B-16468 and generation of new nocamycin derivatives by manipulating gene cluster

Strains or plasmids Description Reference or source
Strains
 E. coli LE392 Host strain of cosmid vector SuperCos I Stratagene
 E. coli DH5α Host strain for general clone Stratagene
 E. coli ET12567/pUZ8002 Host strain for conjugation [22]
 E. coli BW25113 Host strain for PCR-targeting [23]
 S. syringae Nocamycin-producing strain NRRL
 S. syringae MoS1001 ncmB deletion mutant strains originated from Saccharothrix syringae This study
 S. syringae MoS1002 ncmL deletion mutant strains originated from Saccharothrix syringae This study
 S. syringae MoS1003 ncmG deletion mutant strains originated from Saccharothrix syringae This study
Plasmids
 SuperCosI Ampr, Kanr, cosmid vector Stratagene
 pIJ790 Cmlr, including λ-RED (gam, bet, exo) for PCR-targeting [24]
 pIJ773 Aprr, source of acc(3)IV and oriT fragment [24]
 pUZ8002 Kanr, including tra for conjugation [25]
 p5-C-9 Ampr, Kanr, harboring ncmLgene This study
 p2-H-12 Ampr, Kanr, harboring ncmG gene This study