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Table 1 Bacteria and plasmids used in this study

From: Identification of nocamycin biosynthetic gene cluster from Saccharothrix syringae NRRL B-16468 and generation of new nocamycin derivatives by manipulating gene cluster

Strains or plasmids

Description

Reference or source

Strains

 E. coli LE392

Host strain of cosmid vector SuperCos I

Stratagene

 E. coli DH5α

Host strain for general clone

Stratagene

 E. coli ET12567/pUZ8002

Host strain for conjugation

[22]

 E. coli BW25113

Host strain for PCR-targeting

[23]

 S. syringae

Nocamycin-producing strain

NRRL

 S. syringae MoS1001

ncmB deletion mutant strains originated from Saccharothrix syringae

This study

 S. syringae MoS1002

ncmL deletion mutant strains originated from Saccharothrix syringae

This study

 S. syringae MoS1003

ncmG deletion mutant strains originated from Saccharothrix syringae

This study

Plasmids

 SuperCosI

Ampr, Kanr, cosmid vector

Stratagene

 pIJ790

Cmlr, including λ-RED (gam, bet, exo) for PCR-targeting

[24]

 pIJ773

Aprr, source of acc(3)IV and oriT fragment

[24]

 pUZ8002

Kanr, including tra for conjugation

[25]

 p5-C-9

Ampr, Kanr, harboring ncmLgene

This study

 p2-H-12

Ampr, Kanr, harboring ncmG gene

This study