Strains or plasmids | Description | Reference or source |
---|---|---|
Strains | ||
 E. coli LE392 | Host strain of cosmid vector SuperCos I | Stratagene |
 E. coli DH5α | Host strain for general clone | Stratagene |
 E. coli ET12567/pUZ8002 | Host strain for conjugation | [22] |
 E. coli BW25113 | Host strain for PCR-targeting | [23] |
 S. syringae | Nocamycin-producing strain | NRRL |
 S. syringae MoS1001 | ncmB deletion mutant strains originated from Saccharothrix syringae | This study |
 S. syringae MoS1002 | ncmL deletion mutant strains originated from Saccharothrix syringae | This study |
 S. syringae MoS1003 | ncmG deletion mutant strains originated from Saccharothrix syringae | This study |
Plasmids | ||
 SuperCosI | Ampr, Kanr, cosmid vector | Stratagene |
 pIJ790 | Cmlr, including λ-RED (gam, bet, exo) for PCR-targeting | [24] |
 pIJ773 | Aprr, source of acc(3)IV and oriT fragment | [24] |
 pUZ8002 | Kanr, including tra for conjugation | [25] |
 p5-C-9 | Ampr, Kanr, harboring ncmLgene | This study |
 p2-H-12 | Ampr, Kanr, harboring ncmG gene | This study |