Table 2 Primers used in this work
From: Multicopy plasmid integration in Komagataella phaffii mediated by a defective auxotrophic marker
Primer | Sequence (5′→3′)* | Restriction site |
|---|---|---|
PpLEU2-F1 | CAGCTGAAGAGTCCAAGTCCAAG | PvuII |
PpLEU2-R2 | CAGCTGGTGCCATTGGTGGTACTGT | PvuII |
TEF-2F | ATACCTAGGCCCCACACACCATAGCTTCAA | AvrII |
TEF-2R | ATACCTAGGTTTGTAATTAAAACTTAGATTAGATTG | AvrII |
ZeoBlas-F3 | CGGATCCATAACTTCGTATAATGTATGCTATACGAAGTTATAGATCTCCCACACACCATAGCTTCAAAATG | BamHI and BglII |
ZeoBlas-R3 | CGGATCCATAACTTCGTATAGCATACATTATACGAAGTTATAGATCTAGCTTGCAAATTAAAGCCTTCGAG | BamHI and BglII |
PpLEU2-EXT1 | GAGGATAAGCTGGGAGACTATG | – |
PpLEU2-EXT2 | TCTGTTGCCTAAGACTGAGAGC | – |
5-leud | GAGATCTATATATATTTCAAGGATATACCATTCTAATG | BglII |
3-leud | GAGATCTGTTTCATGATTTTCTGTTACACC | BglII |