Fig. 5
From: Multicopy plasmid integration in Komagataella phaffii mediated by a defective auxotrophic marker
Vector copy number determination. a Schematic representation of genomic contexts and expected sizes of bands obtained after hybridization with a PGK probe (annealing positions are represented by a red line). Dark grey areas correspond to integrated P PGK1 sequences derived from vector. b Result of Southern blot analysis. c Correlation between copy number and maximum growth rates of selected clones. Error bars represent standard error of the mean. M O’GeneRuler 1 kb DNA ladder, M12 K. phaffii leu2 strain, E EcoRI restriction site