Fig. 2
From: Multicopy plasmid integration in Komagataella phaffii mediated by a defective auxotrophic marker
Heterologous expression in K. phaffii M12. Intracellular expression of enhanced green fluorescent protein (EGFP) was visualized by epifluorescence microscopy. Untransformed (M12) and transformed (M12 + GFP) cells were viewed under differential interference contrast (DIC) or fluorescence (FM) microscopy after growth in glycerol or methanol containing media. All images were collected with the same exposure time