Fig. 1
From: Multicopy plasmid integration in Komagataella phaffii mediated by a defective auxotrophic marker
Strain phenotypic analysis. Strains LK and M12 were grown in different media to confirm drug resistance and leucine assimilation. X-33 was used as a control. a YPD containing G418 or hygromycin B (Hyg B). b MD lacking or not leucine in different concentrations. c MD with leucine replacing ammonium sulfate. d Buffered MD (pH 6.0) containing leucine. X-33 wild-type strain with intact LEU2 gene, LK leu2 strain disrupted with kan cassette, M12 strain obtained after marker removal with CreA recombinase