Table 1 Strains and plasmids used in this study
Strains and plasmids | Relevant genotype and characteristics | Source |
|---|---|---|
Strains | ||
B. amyloliquefaciens NK-1 | LL3 derivative, ΔpMC1, Δupp | [18] |
B. amyloliquefaciens NK-1-Δstpa | NK-1 derivative, ΔsacTPA | This work |
B. amyloliquefaciens Δ1 | NK-1 derivative, ΔsacA | This work |
B. amyloliquefaciens Δ2 | NK-1 derivative, ΔsacA, ΔsacBlevB | This work |
B. amyloliquefaciens Δ3 | NK-1 derivative, ΔsacA, ΔsacBlevB, ΔRBAM_031820 | This work |
B. amyloliquefaciens Δ1-CES | Δ1 derivative with expression plasmid pWH1520-CES | This work |
B. amyloliquefaciens Δ1-CEG | Δ1 derivative with expression plasmid pWH1520-CEG | This work |
B. amyloliquefaciens Δ1-CBS | Δ1 derivative with expression plasmid pWH1520-CBS | This work |
B. amyloliquefaciens Δ1-CBG | Δ1 derivative with expression plasmid pWH1520-CBG | This work |
B. amyloliquefaciens Δ2-CES | Δ2 derivative with expression plasmid pWH1520-CES | This work |
B. amyloliquefaciens Δ2-CEG | Δ2 derivative with expression plasmid pWH1520-CEG | This work |
B. amyloliquefaciens Δ2-CBS | Δ2 derivative with expression plasmid pWH1520-CBS | This work |
B. amyloliquefaciens Δ2-CBG | Δ2 derivative with expression plasmid pWH1520-CBG | This work |
B. amyloliquefaciens Δ3-CES | Δ3 derivative with expression plasmid pWH1520-CES | This work |
B. amyloliquefaciens Δ3-CEG | Δ3 derivative with expression plasmid pWH1520-CEG | This work |
B. amyloliquefaciens Δ3-CBS | Δ3 derivative with expression plasmid pWH1520-CBS | This work |
B. amyloliquefaciens Δ3-CBG | Δ3 derivative with expression plasmid pWH1520-CBG | This work |
E. coli DH5α | F−, φ80dlacZΔM1, Δ(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(r −k , m +k ), phoA, supE44, λ− thi-1, gyrA96, relA1 | Lab stock |
E. coli GM2163 | F−, ara-14 leuB6 thi-1 fhuA31 lacY1 tsx-78 galK2 galT22 supE44 hisG4 rpsL 136 (Str r ) xyl-5 mtl-1 dam13::Tn9 (Camr) dcm-6 mcrB1 hsdR2 mcrA | Lab stock |
Plasmids | ||
p-KSU | pKSV7-derivation with upp gene | [20] |
pKSV7-ΔsacA | p-KSU-derivation with deletion fragment of sacA | This work |
pKSV7-Δsac | p-KSU-derivation with deletion fragment of sac operon | [33] |
pKSV7-ΔRBAM_031820 | p-KSU-derivation with deletion fragment of RBAM_031820 | This work |
pKSV7-ΔsacTPA | ||
pWH1520 | Tcr; xylose inducible expression vector for Bacillus | MoBiTec |
pWH1520-CES | pWH1520 derivative with genes cscB (E. coli) and sucP | This work |
pWH1520-CEG | pWH1520 derivative with genes cscB (E. coli) and gtfA | This work |
pWH1520-CBS | pWH1520 derivative with genes cscB (B. lactis) and sucP | This work |
pWH1520-CBG | pWH1520 derivative with genes cscB (B. lactisi) and gtfA | This work |