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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Construction of energy-conserving sucrose utilization pathways for improving poly-γ-glutamic acid production in Bacillus amyloliquefaciens

Fig. 2

Schematic for the replacement of the sucrose utilization pathway in Bacillus amyloliquefaciens NK-1 strain. a The native sucrose utilization pathway in NK-1; b the to-be-introduced heterologous energy-conserving sucrose utilization pathway. sacP, PTS sucrose-specific enzyme IIBC component; ptsG, PTS glucose-specific EIIA component; ptsH, phosphocarrier protein HPr; ptsI, PTS enzyme I; sacA, sucrase-6-phosphate hydrolase; RBAM_031820, sucrase-6-phosphate hydrolase; pyk, pyruvate kinase; cscB, sucrose permease; sucP, sucrose phosphorylase; gtfA, sucrose phosphorylase; fruK, fructose kinase; pgcA, phosphoglucomutase; ldh, lactic dehydrogenase; pta, phosphotransacetylase; pfhABC, pyruvate dehydrogenase; ackA, acetate kinase. The purple words indicated the metabolites measured in this work

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