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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Improving the production of 22-hydroxy-23,24-bisnorchol-4-ene-3-one from sterols in Mycobacterium neoaurum by increasing cell permeability and modifying multiple genes

Fig. 5

Enhancement of the 4-HBC productivity by overexpressing the genes in the sterol conversion pathway. a Assessment of the 4-HBC production for overexpressing the genes in the upstream conversion pathway of sterol to 4-HBC. b Growth of the WIIIΔmmpL3-cyp125-choM1-fadA5 (co-overexpression of cyp125, choM1, and fadA5 in the strain WIIIΔmmpL3) and its ancestral strain WIII in MYC/02 medium with 2.0 g/L phytosterol. c Assessment of the 4-HBC production in the constructed 4-HBC-producing strains. A “cyclodextrin-resting cell” system with 20 g/L of phytosterol was used to determine the productivity of the engineered strains. Data represent the mean ± standard deviation of three measurements

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Microbial Cell Factories

ISSN: 1475-2859

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