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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Improving the production of 22-hydroxy-23,24-bisnorchol-4-ene-3-one from sterols in Mycobacterium neoaurum by increasing cell permeability and modifying multiple genes

Fig. 4

Effects of deleting mmpL3 on the improvement in 4-HBC production in the engineered mycobacterial strains. a Improvement in cell permeability in the mmpL3-deleted strain. The engineered strains were stained with FDA, incubated at 32 °C for 10 min and then the mixtures were analyzed by the fluorescence spectrophotometer. b Growth of the previously constructed 4-HBC-producing strain (WIII) and the mmpL3-deleted strain WIIIΔmmpL3 in MYC/02 medium with 2.0 g/L phytosterol. CFU colony forming unit. c Quantitative analyses of the 4-HBC production of the aerobic bioconversion by 4-HBC-producing strains in MYC/02 medium with 2.0 g/L phytosterol. Data represent the mean ± standard deviation of three measurements

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Microbial Cell Factories

ISSN: 1475-2859

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