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Table 5 Estimated distribution of TCA cycle acids during the fed-batch process (t = 52 h and t = 73 h)

From: Intracellular product recycling in high succinic acid producing yeast at low pH

 

% Amount cytosol 52 h (%)

% Amount cytosol 73 h (%)

Conc. ratio M/C 52 h

Conc. ratio M/C 73 h

αKG

40.0

40.0

15.0

15.0

Cit

94.9

96.6

0.5

0.3

FUM

40.0

95.5

15.0

0.5

iCit

62.5

78.2

6.0

2.8

Mal

99.6

99.8

0.0

0.0

Oaa

54.7

89.9

8.3

1.1

Suc

99.8

99.8

0.0

0.0

  1. Please note, the concentration ratio depends on assumptions for the subcellular volumes (here cytosol 70%, mitochondria 7%)