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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Efficient extracellular recombinant production and purification of a Bacillus cyclodextrin glucanotransferase in Escherichia coli

Fig. 3

SDS-PAGE analysis of proteins in the extracellular fractions of cultures expressing CGTase genes with different SP. Lane 1 CGTase without SP; lane 2 CgtS-CGTase; lane 3 PelB-CGTase; lane 4 DacD-CGTase; lane 5 DacD-CGTase purified by batchwise starch adsorption; lane 6: DacD-CGTase obtained with 100 mM glycine added to the culture medium. M molecular mass marker. 10 µg protein for extracellular fractions and 1 µg of purified protein was loaded. CGTase bands are marked with an asterisk

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