Table 1 Plasmids used in this study
From: Chromosome engineering of Escherichia coli for constitutive production of salvianic acid A
Plasmids | Characteristics | Source |
|---|---|---|
pKD3 | FRT (FLP recognition target) sites; CmR | [32] |
pKD46 | Red recombinase expression vector; AmpR | [32] |
pCP20 | FLP expression vector; AmpR, CmR | [32] |
pACYCDuet-1 | p15A ori; CmR | Novagen |
pYBT5 | pBldgbrick 1 with P lacUV5 -aroG fbr-tyrA fbr-aroE, P trc -ppsA-tktA-glk | [12] |
pYBD4 | pCDFDuet-1 with hpaBC and d-ldh Y52A | [12] |
pZL | p15A ori; CmR | This study |
pZL1 | pZL with fragment 1 | This study |
pZL2 | pZL with fragment 2 | This study |
pZL3 | pZL with P BBa-J23100 -hpaBC and P BBa-J23100 -d-ldh Y52A | This study |
pZL4 | pZL with P tac -hpaBC and P tac -d-ldh Y52A | This study |
pZL5 | pZL with P tac -hpaBC and P BBa-J23100 -d-ldh Y52A | This study |
pZL6 | pZL with P BBa-J23100 -hpaBC and P tac -d-ldh Y52A | This study |
pZL7 | pZL with fragment 3 | This study |
pZL8 | pZL7 with rrnB P1 promoter | This study |
pZL9 | pZL with P 5tacs -hpaBC- d-ldh Y52A | This study |
pZL10 | pZL with P rrnB P1 -hpaBC- d-ldh Y52A | This study |