Fig. 2
From: Chromosome engineering of Escherichia coli for constitutive production of salvianic acid A
Construction of plasmid-free chassis strain BAK11 to produce l-tyrosine constitutively. a Replacement of the mao-paa cluster with module 1. Lane 1 The paaZ gene of the mao-paa cluster in strain BAK5 with primers paaZ F/R; lane 2 ydbL-M1-Chl-ydbA fragment in strain BAK10-1 with primers ydbL F and ydbA R; lane 3 ydbL-M1-ydbA fragment in strain BAK10 with primers ydbL F and ydbA R; M: DNA marker. b Cell growth and l-tyrosine production of strains BAK5 and BAK10. 0.1Â mM IPTG was added into the medium when needed. c Replacement of the lacI gene with module 2. Lane 1 ydbL-lacI-ydbA in strain BAK10; lane 2 ydbL-M2-Chl-ydbA fragment in strain BAK11-1; lane 3 ydbL-M2-ydbA fragment in strain BAK11; primers lacZ F and mhpR R were used for all PCR verification. M: DNA marker. d Cell growth and l-tyrosine production of strain BAK11. 0.1Â mM IPTG was added into the medium when needed