Fig. 1
From: Chromosome engineering of Escherichia coli for constitutive production of salvianic acid A
Chromosomal engineering for constitutive production of salvianic acid A (SAA) in E. coli. a The artificial synthetic pathway of SAA from glucose. b The targeted integration of three modules into chromosome of engineered strains. In-frame deletion of the ptsG, pykF, pykA, pheA and tyrR genes were not shown in chromosome of strain BAK5. 6‐P‐G 6‐phosphate‐glucose, 6‐P‐F 6‐phosphate‐fructose, E4P erythrose‐4‐phosphate, PEP phosphoenolpyruvate, PYR pyruvic acid, DAHP 3‐deoxy‐d‐arabino‐heptulosonate‐7‐phosphate, DHS 3‐dehydroshikimic acid, SHK shikimic acid, CHA chorismic acid, l -Phe l-phenylalanine, 4‐HPP 4‐hydroxyphenylpyruvic acid, l -Tyr l-tyrosine, DHPP 3,4-Dihydroxyphenylpyruvate, 4-HPL 4-Hydroxyphenyllactate, TyrR-tyr TyrR-tyrosine DNA-binding transcriptional repressor, SAA salvianic acid A