Table 3 Cellular sterol composition
From: The effect of hypoxia on the lipidome of recombinant Pichia pastoris
Culture conditions | µg sterol/mg total protein | |||||||
|---|---|---|---|---|---|---|---|---|
Squalene | Lanosterol | Ergostadienol | 4-Methyl zymosterol | Zymosterol | Fecosterol | Episterol | Ergosterol | |
Normoxia | n.d. | n.d. | n.d. | 0.89 ± 0.36 | 0.75 ± 0.44 | n.d. | 0.17 ± 0.05 | 8.84 ± 1.98 |
Hypoxia | n.d. | 0.14 ± 0.00 | n.d. | 0.80 ± 0.18 | 0.52 ± 0.23 | n.d. | 0.38 ± 0.19 | 6.21 ± 1.20 |
Fluconazole | n.d. | 1.39 ± 0.51* | 0.21 ± 0.06 | n.d. | 0.10 ± 0.05 | 0.78 ± 0.39 | 0.18 ± 0.05 | 6.43 ± 1.24 |
Fluconazole + hypoxia | 0.18 ± 0.07 | 5.06 ± 0.57*†| 0.77 ± 0.35 | n.d. | 0.58 ± 0.41 | 0.90 ± 0.25 | 0.94 ± 0.47*†| 7.71 ± 1.54 |