Fig. 7From: Directed evolution and secretory expression of a pyrethroid-hydrolyzing esterase with enhanced catalytic activity and thermostabilityEffect of temperature on stability of the wild-type enzyme (black), M1 (red), M2 (blue) and M3 (dark cyan). Thermostability was measured by preincubation of the purified enzymes in 50 mM potassium phosphate buffer (pH 6.5) at 45–65 °C for 12 h. Data points are the average of triplicate measurements, and error bars represent the standard deviationBack to article page