Fig. 1From: Directed evolution and secretory expression of a pyrethroid-hydrolyzing esterase with enhanced catalytic activity and thermostabilityScreening of mutants with better activity (→). The transformants were replicated on LB agar plates supplemented with 0.1% (v/v) tributyrin. A transparent zone due to tributyrin hydrolysis appeared at 37 °C in 1–2 days. Clones with enhanced activity displayed bigger hydrolysis zones on tributyrin platesBack to article page