Fig. 3

The purification, western blot analysis and secondary folding of recombinant ThSwo. a SDS-PAGE (12%) and b western blot analysis of ThSwo using a His-probe antibody. M molecular mass markers, in both figures a and b; lane 1 cell lysate before protein induction; lane 2 cell lysate after protein induction; lane 3 protein eluted from nickel affinity chromatography; and lane 4 proteins eluted from size-exclusion chromatography. c Net-smoothed CD spectrum of ThSwo. d ThSwo thermal stability followed by CD. The ThSwo thermal-induced unfolding experiments were measured at 218 nm from 20 to 90 °C using approximately 10 µM of purified recombinant ThSwo in buffer A containing 1 mM of TCEP and with a 1 mm pathlength cell. ThSwo had a Tm of 48.6 ± 2 °C