Production of fuels and chemicals from xylose by engineered Saccharomyces cerevisiae: a review and perspective

Table 1 Comparison of performances of representative xylose-fermenting engineered S. cerevisiae

Strains	Strain descriptions		Culture conditions	Xylose consumption rate	Ethanol production rate	Ethanol yield (g/g xylose)	Reference
H131-A3-AL^CS	XI (Piromyces XYLA)	SsXYL3, SsTAL1, TKL1, RPE1, RKI1	Anaerobic batch, 2× YNB, 4% xylose	1.866 (g g⁻¹ h⁻¹)	0.765 (g g⁻¹ h⁻¹)	0.410	[44]
SXA-R2P-E	XI (mutant Piromyces XYLA)	SsTAL1, Δgre3, Δpho13	Anaerobic batch, YNB, 4% xylose	0.077(g OD⁻¹ h⁻¹)	0.033 (g OD⁻¹ h⁻¹)	0.453	[61, 95]
SR8	XR–XDH (SsXYL1, SsXYL2)	SsXYL3, Δald6, null mutant of PHO13	Anaerobic batch, YNB, 4% xylose	0.129 (g OD⁻¹ h⁻¹)	0.046 (g OD⁻¹ h⁻¹)	0.378	[57, 61]
TMB 3422	XR–XDH (mutant SsXYL1, SsXYL2)	XKS1, TAL1, TKL1, RPE1, RKI1, Δgre3	Anaerobic batch, 2× YNB, 5% xylose	0.580 (g g⁻¹ h⁻¹)	0.180 (g g⁻¹ h⁻¹)	0.340	[74, 150]
TMB 3504	XR–XDH (SpXYL1.2, SsXYL2)	XKS1, TAL1, TKL1, RPE1, RKI1, Δgre3	Anaerobic batch, 2× YNB, 5% xylose	0.760 (g g⁻¹ h⁻¹)	0.330 (g g⁻¹ h⁻¹)	0.400	[74]

ISSN: 1475-2859