Fig. 1

Tetracladium sp. amylase substrate specificity. a Amylase assays were performed at 30 °C using ethylidene-pNP-G7 (open symbols) or 4-nitrophenyl α-d-glucopyranoside (filled symbols) as substrates. The release of p-nitrophenol was measured by absorbance at 405 nm, which was quantified using a calibration curve previously constructed with pure p-nitrophenol solutions. Results for the Tetracladium sp. amylase are shown as squares, commercial α-amylase by open circles, commercial alpha-glucosidase by filled circles and the negative control (no enzyme) by triangles. b Assays with the Tetracladium sp. amylase were performed using 10 g/L of soluble starch as substrate at pH 6.0 and the release of glucose was followed by the DNS method