Fig. 1
From: Heterologous expression of abaecin peptide from Apis mellifera in Pichia pastoris
Electrophoresis Tricine-SDS-PAGE, silver-stained. Expression time: T1 (24 h), T2 (48 h), T3 (72 h), T4 (96 h) with addition of 0.5% of methanol at all times. It shows the production of the peptide abaecin, of approximately 5.2 kDa, migrating lower than the last band of the marker (M) of 6.5 kDa. The beginning of the peptide’s expression happened at the 72 h of incubation under 30 °C