Table 1 The strains and plasmids used in this study

Escherichia coli

DH5α

supE44 ΔlacU169 (f 80 lacZΔM15) hsd R17 recA1 gyrA96 thi1 relA1

[7]

Bacillus licheniformis

WX-02

Polyglutamate productive strain (CCTCC M208065)

CCTCC

BL10

WX-02(Δmpr, Δvpr, ΔaprX, Δepr, Δbpr, ΔwprA, ΔaprE, ΔbprA, Δhag, ΔamyL)

[7]

BL10/pHY-amyL

BL10 harboring pHY-amyL

This study

BL10/pP43SacCNK

BL10 harboring pP43SacCNK(CCTCC M2014253)

CCTCC

BL10T

BL10(ΔtepA)

This study

BL10S

BL10(ΔsppA)

This study

BL10T/pHY-amyL

BL10T harboring pHY-amyL

This study

BL10S/pHY-amyL

BL10S harboring pHY-amyL

This study

BL10T/pP43SacCNK

BL10T harboring pP43SacCNK

This study

BL10S/pP43SacCNK

BL10S harboring pP43SacCNK

This study

BL10GT

Overexpression of tepA in BL10

This study

BL10GS

Overexpression of sppA in BL10

This study

BL10GT/pHY-amyL

BL10GT harboring pHY-amyL

This study

BL10GS/pHY-amyL

BL10GS harboring pHY-amyL

This study

BL10GT/pP43SacCNK

BL10GT pP43SacCNK

This study

BL10GS/pP43SacCNK

BL10GS pP43SacCNK

This study

Plasmids

This study

T₂(2)-ori

Bacillus knockout vector; Kan^r

[7]

T₂-sppA

T2(ori)-sppA(A + B); to knock out sppA

This study

T₂-tepA

T2(ori)-tepA(A + B); to knock out tepA

This study

T₂-GsppA

T2(ori)-sppA(A + B+sppA); to over-express sppA

This study

T₂-GtepA

T2(ori)-tepA(A + B+tepA); to over-express tepA

This study

pHY300PLK

E. coli and B. s shuttle vector; Amp^r, Tet^r

[7]

pP43SacCNK

PHY300PLK + Promotor-P43 (B. subtilis 168) + signal peptide of SacC (B. subtilis 168) + aprN(B. subtilis MBS 04-6) + Terminator of amyL (B. licheniformis WX-02)

[7]

pHY-amyL

PHY300PLK + Promotor-P43 (B. subtilis 168) + amyL (containing its own signal peptide and terminator, B. licheniformis WX-02)

This study