Fig. 5

Flow cytometric analysis of Lactobacillus adherence to Caco-2 cells. a Autofluorescence of Caco-2 cells. b Caco-2 cells exposed to fluorescently labeled L. reuteri Pg4. c Caco-2 cells exposed to fluorescently labeled L. reuteri pNZ3004. d Caco-2 cells exposed to fluorescently labeled L. reuteri pNZ-zhd101. Bacterial cells were labeled with hexidium iodide (HI) and incubated with Caco-2 cells for 2 h. For each experiment, 10,000 Caco-2 cells were analyzed. The red fluorescence intensity of the Caco-2 cells with adherent lactobacilli was measured by flow cytometry at 620 nm emission wavelengths. Mean red fluorescence intensity (MFI) was calculated from two independent experiments performed in triplicate