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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Expression of the Clonostachys rosea lactonohydrolase gene by Lactobacillus reuteri to increase its zearalenone-removing ability

Fig. 2

Expression of Zhd101 in Lactobacillus reuteri cells. a RT-PCR for Zhd101 in Lb. reuteri Pg4, Lb. reuteri pNZ-zhd101, and Lb. reuteri pNZ3004, along with the housekeeping gene 16S rRNA. b Western blot for purified recombinant Zhd101 and Zhd101 in the intracellular extracts of Lb. reuteri Pg4, Lb. reuteri pNZ3004, and Lb. reuteri pNZ-zhd101. The samples (2 μg of protein in each lane) were separated by SDS-PAGE with a 12.5% gel and probed with mouse polyclonal anti-zhd101 antibody and horseradish-peroxidase-linked anti-mouse IgG antibody as the primary and secondary antibodies, respectively

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Microbial Cell Factories

ISSN: 1475-2859

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