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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Direct expression of active human tissue inhibitors of metalloproteinases by periplasmic secretion in Escherichia coli

Fig. 3

Size exclusion chromatography of purified a N-TIMP-2 and b N-TIMP-1. The size-exclusion column was equilibrated with 50 mM HEPES (pH 7.5) and 150 mM NaCl. 100 µL of 500 µg/mL N-TIMP-1/-2 was loaded to a superdex™ 75 10/300 GL column (10 mm × 300 mm) at a flow rate of 0.5 mL/min. Chromatograms were obtained by monitoring absorbance at 280 nm. The molecular mass of N-TIMP-1/-2 was estimated by its retention time and comparison with these of standard molecular mass markers, e.g. ovalbumin (43 kDa) and lysozyme (14.3 kDa)

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